Critical design issues for gallium arsenide VLSI circuits.

The aim of this research was to design and evaluate various Gallium Arsenide circuit elements such as logic gates, adders and multipliers suitable for high speed VLSI circuits. The issues addressed are the logic gate design and optimisation, evaluation of various buffering schemes and the impact of the algorithm on adder and multiplier performance for digital signal processing applications. This has led to the development of a design approach to produce high speed and low power dissipation Gallium Arsenide VLSI circuits. This is achieved by : Evaluating the well established Direct Coupled Logic (DCFL) gates and proposing an alternative gate, namely the Source Follower DCFL (SDCFL), to improve the noise margin and speed. Suggesting various buffering schemes to maintain high speed in areas where the fanout loading is high (eg. clock drivers). Comparing various adder types in terms of delay-power and delay-area products to arrive at a suitable architecture for Gallium Arsenide implementation and to determine the influence of the algorithm and layout approach on circuit performance. To investigate this further, a multiplier was also designed to assess the performance at higher levels of integration. Applying a new layout approach, called the 'ring notation*, to the adder and multiplier circuits in order to improve their delay-area product. Finally, the critical factors influencing the performance of the circuits are reviewed and a number of suggestions are given to maintain reliable operation at high speed

The Antifungal Eugenol Perturbs Dual Aromatic and Branched-Chain Amino Acid Permeases in the Cytoplasmic Membrane of Yeast

Eugenol is an aromatic component of clove oil that has therapeutic potential as an antifungal drug, although its mode of action and precise cellular target(s) remain ambiguous. To address this knowledge gap, a chemical-genetic profile analysis of eugenol was done using ∼4700 haploid Saccharomyces cerevisiae gene deletion mutants to reveal 21 deletion mutants with the greatest degree of susceptibility. Cellular roles of deleted genes in the most susceptible mutants indicate that the main targets for eugenol include pathways involved in biosynthesis and transport of aromatic and branched-chain amino acids. Follow-up analyses showed inhibitory effects of eugenol on amino acid permeases in the yeast cytoplasmic membrane. Furthermore, phenotypic suppression analysis revealed that eugenol interferes with two permeases, Tat1p and Gap1p, which are both involved in dual transport of aromatic and branched-chain amino acids through the yeast cytoplasmic membrane. Perturbation of cytoplasmic permeases represents a novel antifungal target and may explain previous observations that exposure to eugenol results in leakage of cell contents. Eugenol exposure may also contribute to amino acid starvation and thus holds promise as an anticancer therapeutic drug. Finally, this study provides further evidence of the usefulness of the yeast Gene Deletion Array approach in uncovering the mode of action of natural health products